Journal: Scientific Reports

Article Title: Real-time monitoring of the dynamics and interactions of bacteria and the early-stage formation of biofilms

doi: 10.1038/s41598-022-22669-0

Figure Lengend Snippet: Comparison between the same E. coli bacteria population imaged with an inverted optical microscope in caustics mode (top) and fluorescence mode (bottom). E. coli bacteria were stained with the fluorescent dyes SYTO9 and propidium iodide (PI) from the LIVE/DEAD BacLight kit. There are no significant differences between the two imaging techniques. The three bacteria highlighted by the black arrows in the caustics image are not at the surface and have been captured when aligned perpendicularly to the surface during their random diffusion, resulting in an almost spherical optical signature.

Article Snippet: Bacterial dynamics and interactions were monitored in a standard optical inverted microscope (Axio Observer.Z1 m, Carl Zeiss), mounted on antivibration feet (VIBe, Newport) to isolate the sample from the environment, using 60 μl of bacteria solution in a deep cavity (250 ± 10 μm in depth) in microscopy slides.

Techniques: Comparison, Bacteria, Microscopy, Fluorescence, Staining, Imaging, Diffusion-based Assay

Journal: Scientific Reports

Article Title: Real-time monitoring of the dynamics and interactions of bacteria and the early-stage formation of biofilms

doi: 10.1038/s41598-022-22669-0

Figure Lengend Snippet: Photograph of the optical signature generated by an E. coli bacterium taken with the optical inverted microscope used in this work set-up for: ( a ) caustics mode; ( b ) brightfield mode.

Article Snippet: Bacterial dynamics and interactions were monitored in a standard optical inverted microscope (Axio Observer.Z1 m, Carl Zeiss), mounted on antivibration feet (VIBe, Newport) to isolate the sample from the environment, using 60 μl of bacteria solution in a deep cavity (250 ± 10 μm in depth) in microscopy slides.

Techniques: Generated, Inverted Microscopy

Journal: Scientific Reports

Article Title: Real-time monitoring of the dynamics and interactions of bacteria and the early-stage formation of biofilms

doi: 10.1038/s41598-022-22669-0

Figure Lengend Snippet: E. coli bacteria stained with trypan blue and statically attached to a BKC surface imaged with an inverted optical microscope in caustics mode ( a ) and fluorescence mode ( b ).

Article Snippet: Bacterial dynamics and interactions were monitored in a standard optical inverted microscope (Axio Observer.Z1 m, Carl Zeiss), mounted on antivibration feet (VIBe, Newport) to isolate the sample from the environment, using 60 μl of bacteria solution in a deep cavity (250 ± 10 μm in depth) in microscopy slides.

Techniques: Bacteria, Staining, Microscopy, Fluorescence